membrite fix cell surface staining kit Search Results


membrite fix cell surface staining kit  (Biotium)
93
Biotium membrite fix cell surface staining kit
CXCL12 and HMGB1 are expressed in MCF-7, MDA-MB-231, and PC-3 cancer cell lines. (A, B) qRT-PCR analysis (normalized on 18S ) is shown for CXCL12 and HMGB1 mRNA in cancer cells. Data are shown as mean ± SEM of 5 independent experiments. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: **p < 0.01. (C) CXCL12 and HMGB1 expression assessed by confocal microscopy. In the merged images, CXCL12 is shown in red and HMGB1 in green. Nuclei and cell membranes were counterstained with DAPI (blue) and <t>MemBrite</t> Fix Dye (cyan), respectively. Representative images, of at least 3 independent experiments, are shown. Scale bar represents 20 µm. (D, E) CXCL12 (D) and HMGB1 (E) expression was evaluated in at least 40 cells and expressed as fluorescence intensity. Horizontal lines represent means. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05, ****p < 0.0001. (F) Confocal microscope z-stack overlays showing the intracellular localization of CXCL12 in MCF-7 cells (scale bar 20 μm). CXCL12 is shown in red, while nuclei were counterstained with DAPI (blue) and cell membranes with the MemBrite Fix Dye (cyan).
Membrite Fix Cell Surface Staining Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/membrite fix cell surface staining kit/product/Biotium
Average 93 stars, based on 1 article reviews
membrite fix cell surface staining kit - by Bioz Stars, 2026-03
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CXCL12 and HMGB1 are expressed in MCF-7, MDA-MB-231, and PC-3 cancer cell lines. (A, B) qRT-PCR analysis (normalized on 18S ) is shown for CXCL12 and HMGB1 mRNA in cancer cells. Data are shown as mean ± SEM of 5 independent experiments. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: **p < 0.01. (C) CXCL12 and HMGB1 expression assessed by confocal microscopy. In the merged images, CXCL12 is shown in red and HMGB1 in green. Nuclei and cell membranes were counterstained with DAPI (blue) and MemBrite Fix Dye (cyan), respectively. Representative images, of at least 3 independent experiments, are shown. Scale bar represents 20 µm. (D, E) CXCL12 (D) and HMGB1 (E) expression was evaluated in at least 40 cells and expressed as fluorescence intensity. Horizontal lines represent means. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05, ****p < 0.0001. (F) Confocal microscope z-stack overlays showing the intracellular localization of CXCL12 in MCF-7 cells (scale bar 20 μm). CXCL12 is shown in red, while nuclei were counterstained with DAPI (blue) and cell membranes with the MemBrite Fix Dye (cyan).

Journal: Frontiers in Immunology

Article Title: Tumor cells express and maintain HMGB1 in the reduced isoform to enhance CXCR4-mediated migration

doi: 10.3389/fimmu.2024.1358800

Figure Lengend Snippet: CXCL12 and HMGB1 are expressed in MCF-7, MDA-MB-231, and PC-3 cancer cell lines. (A, B) qRT-PCR analysis (normalized on 18S ) is shown for CXCL12 and HMGB1 mRNA in cancer cells. Data are shown as mean ± SEM of 5 independent experiments. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: **p < 0.01. (C) CXCL12 and HMGB1 expression assessed by confocal microscopy. In the merged images, CXCL12 is shown in red and HMGB1 in green. Nuclei and cell membranes were counterstained with DAPI (blue) and MemBrite Fix Dye (cyan), respectively. Representative images, of at least 3 independent experiments, are shown. Scale bar represents 20 µm. (D, E) CXCL12 (D) and HMGB1 (E) expression was evaluated in at least 40 cells and expressed as fluorescence intensity. Horizontal lines represent means. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05, ****p < 0.0001. (F) Confocal microscope z-stack overlays showing the intracellular localization of CXCL12 in MCF-7 cells (scale bar 20 μm). CXCL12 is shown in red, while nuclei were counterstained with DAPI (blue) and cell membranes with the MemBrite Fix Dye (cyan).

Article Snippet: Nuclei were counterstained with 2 mM DAPI (62248, Thermo Fisher Scientific), and the cell membrane was stained using the MemBrite Fix cell surface staining kit from Biotium, following the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Comparison, Expressing, Confocal Microscopy, Fluorescence, Microscopy

CXCL12 uptake by cancer cells. (A) qRT-PCR analysis (normalized on 18S ) is shown for CXCR4 mRNA expression in cancer cells. Data are shown as mean ± SEM of 5 independent experiments. (B) CXCR4 expression analyzed by flow cytometry in MCF-7, MDA-MB-231, and PC-3 cells. Data are shown as relative MFI (rMFI, mean ± SEM) of 3 independent experiments. (C) Uptake of CXCL12-Atto647 by cancer cells analyzed by flow cytometry. Left: A representative histogram showing fluorescence intensity of unstimulated (blue) and CXCL12-Atto647 stimulated cells (red). Right: CXCL12-Atto647 uptake shown as rMFI (mean ± SEM) of 5 independent experiments. (D) Uptake of CXCL12-Atto674 by cancer cells treated with the CXCR4 antagonist AMD3100. Chemokine uptake shown as rMFI (mean ± SEM) of 6 independent experiments. Statistical analysis of the differences among the cell lines was performed using two-way ANOVA, followed by Šídák’s multiple comparisons test: ****p < 0.0001. (E) CXCL12-Atto647 uptake assessed by confocal microscopy in MCF-7, MDA-MB-231, and PC-3 cells. Left: In the merged images, CXCL12 is shown in red. Nuclei and cell membranes were counterstained with DAPI (blue) and MemBrite Fix Dye (cyan), respectively (scale bars: 20 μm). Right: CXCL12-Atto647 uptake was evaluated in at least 10 cells and expressed as fluorescence intensity. Horizontal lines represent the mean values. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05, ****p < 0.0001.

Journal: Frontiers in Immunology

Article Title: Tumor cells express and maintain HMGB1 in the reduced isoform to enhance CXCR4-mediated migration

doi: 10.3389/fimmu.2024.1358800

Figure Lengend Snippet: CXCL12 uptake by cancer cells. (A) qRT-PCR analysis (normalized on 18S ) is shown for CXCR4 mRNA expression in cancer cells. Data are shown as mean ± SEM of 5 independent experiments. (B) CXCR4 expression analyzed by flow cytometry in MCF-7, MDA-MB-231, and PC-3 cells. Data are shown as relative MFI (rMFI, mean ± SEM) of 3 independent experiments. (C) Uptake of CXCL12-Atto647 by cancer cells analyzed by flow cytometry. Left: A representative histogram showing fluorescence intensity of unstimulated (blue) and CXCL12-Atto647 stimulated cells (red). Right: CXCL12-Atto647 uptake shown as rMFI (mean ± SEM) of 5 independent experiments. (D) Uptake of CXCL12-Atto674 by cancer cells treated with the CXCR4 antagonist AMD3100. Chemokine uptake shown as rMFI (mean ± SEM) of 6 independent experiments. Statistical analysis of the differences among the cell lines was performed using two-way ANOVA, followed by Šídák’s multiple comparisons test: ****p < 0.0001. (E) CXCL12-Atto647 uptake assessed by confocal microscopy in MCF-7, MDA-MB-231, and PC-3 cells. Left: In the merged images, CXCL12 is shown in red. Nuclei and cell membranes were counterstained with DAPI (blue) and MemBrite Fix Dye (cyan), respectively (scale bars: 20 μm). Right: CXCL12-Atto647 uptake was evaluated in at least 10 cells and expressed as fluorescence intensity. Horizontal lines represent the mean values. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05, ****p < 0.0001.

Article Snippet: Nuclei were counterstained with 2 mM DAPI (62248, Thermo Fisher Scientific), and the cell membrane was stained using the MemBrite Fix cell surface staining kit from Biotium, following the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Flow Cytometry, Fluorescence, Confocal Microscopy, Comparison